2-3% fetal calf serum in cell media is sufficient for the development and growth of human mononuclear cell cultures

Aim: It is a common practice that 10-20% fetal calf serum (FCS) being used in the cell culture media for development and maintenance of human mononuclear cells as well other cultures. FCS produced through painful process involving animals one major cost factors. The reduction volume can make it less unethical reduce costs strongly. Materials Methods: Therefore 2-3 % 10% were different cultures making comparisons parameters like morphology, production antibodies, isolation DNA/RNA with mini-column magnetic beads isolations along presence internal controls real-time PCR tested. Results: was found there no difference between 10 containing above-said parameters. More than hundred such developed many them maintained more 3 years. Conclusion: sufficient over long period time.